|
Catalog #
|
DMG-2B6-200
|
|
Size
|
200 Reactions
|
|
Store At
|
-20°C
|
|
|
Human cytochrome P450 2B6 (CYP2B6) is a member of
the monooxygenase superfamily of enzymes which catalyze many reac-tions
involved in drug metabolism and synthesis of cholesterol, steroids,
and other lipids. Expression of CYP2B6 is regulated by the vitamin D
receptor pathway in primary human hepatocytes1. In liver microsomes,
this enzyme is involved in an NADPH-dependent electron transport path-way
2. This protein localizes to the endoplasmic reticulum, and its expres-sion
is induced by phenobarbital1. The enzyme is known to metabolize
some xenobiotics, such as the anti-cancer drugs cyclophosphamide and
ifosphamide3. Transcript variants for this gene have been described; how-ever,
it has not been resolved whether these transcripts are produced by
this gene or by a closely related pseudogene, CYP2B73 The CYP2B6 is
genetically polymorphic among individuals and ethnic groups. Genetic
changes can influence enzymatic activity, and the resultant phenotypic
changes are characterized into poor, intermediate, extensive, and ultrarapid
metabolism4. Some variants are known to exhibit reduced kinetics for the
substrates cyclophosphamide and tamoxifen5. Both the gene and the
pseudogene are located in the middle of a CYP2A pseudogene found in
a large cluster of cytochrome P450 genes from the CYP2A, CYP2B and
CYP2F subfamilies on chromosome 19q136. The CYP2B6 gene spans
approximately 27 kb with 9 exons.
|
