| 1. |
Pellet cells in microtiter wells by centrifugation. |
| 2. | Remove culture media without disturbing cell pellet. |
| 3. |
Add 20µl of DI H2O to each well. |
| 4. |
Add 20µl of GeneReleaser® to each wellL. |
| 5. | Mix by vortexing or agitation or up/down pipetting action of the contents of the wells. |
| 6. |
Seal plate. |
| 7. | Float plate on gently boiling water bath for 10 minutes. |
| 8. |
Centrifuge to pellet GeneReleaser® and cell debris. |
| 9. |
Transfer supernatants to fresh plate or use aliquot from prepared plate for PCR. usually 5 - 10µl per 100µl PCR reaction. |
