| 1. | Transfer an aliquot of a suspension of Buccal cells containing ~ 106 nucleated cells to a 0.5 ml amplification tube. |
| 2. | Microfuge the tube for 1 minute at 12,000xg. |
| 3. | Remove and discard the supernatant, leaving the buccal cell pellet in the tube |
| Step 4 and 5 are optional: |
| 4. | Wash the remaining cell pellet 2 X with 200µl of 1x amplification buffer by resuspending the cells in the buffer and repeating the centrifugation step. |
| 5. | Following the last wash step, discard the supernatant as completely as possible. |
| 6. | Add 20µl of GeneReleaser® and proceed with either the thermocycle or microwave lysis protocols. |
