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GeneReleaser® DNA AMPLIFICATION FROM DENTAL PLAQUE Protocol
1. Take a small (~ .1mm3) portion of the plaque and vortex with 100ul normal saline in 0.5ml pcr tube.
2. Microfuge for 1 minute at ~ 14,000 x g.
3. Discard supernatant.
4. Treat pellet per protocol by microwave procedure.
5. Amplify per standard protocol.
6. Serial dilutions of the plaque suspension may be required to establish optimum preparation.

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