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Home :: Technical Resources :: GeneReleaser® Protocols
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Technical Resources
GeneReleaser® MTB SAMPLE PREPARATION Protocol
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MTB SAMPLE PREPARATION
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A. FOR NaOH DECONTAMINATED PREPARATION
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| 1. | Concentrate or enrich MTB by standard method (ASM or other) from
sputum, urine etc. Transfer suitable aliquot to 0.5 ml PCR tube. |
| 2. | If NaOH decontamination method was employed, neutralize excess
NaOH by three washes with 20 volumes (20 x the estimated volume of
the pellet obtained from enrichment) 1M tris buffer pH 8.3. Centrifuge
after each was to repellet the MTB containing material. |
| 3. | Completely remove the supernatant from the last wash. |
| 4. | Treat the remaining pellet with GeneReleaser® according to the microwave procedure and perform PCR. |
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B. FOR ENRICHED BUT NONDECONTAMINATED SAMPLE MATERIAL
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| 1. | Transfer suitable aliquot of enriched MTB sample prior to NaOH decontamination step to 0.5 ml PCR tube. |
| 2. | Treat residue with GeneReleaser® according to microwave protocol and perform PCR. |
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Using either of the above methods we can detect 10 MTB organisms.
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If you have further questions, please contact us at support@bioventures.com. You may also contact us via fax (1-877-286-0330) or through our toll-free phone number (1-877-852-7846).
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