| 1. | Obtain plasma or serum by standard collection techniques. |
| 2. | For maximum sensitivity, treat 5µl of serum with 15µl of resuspended GeneReleaser®. |
| 3. | Vortex the mixture briefly, i.e. 1-2 seconds. |
| 4. | Overlay with mineral oil. |
| 5. | Treat the specimens using either the thermocycle lysis program or by the microwave protocol. |
| 6. | Place samples in thermocycler if you have used the microwave protocol . |
| 7. | Heat to 80°C for 5 minutes . |
| 8. | Add appropriate volume of master mix and begin PCR. (In the above example, this would be 80µl of a 1.25 X master mix.) |
| Note: If serum aggregation occurs (esp. with microwave protocol), we suggest the addition of fetal calf serum at a ratio of 1:1 with your serum. |
