| 1. | The swab should be placed in suitable preservation media (non
inhibitory for PCR). |
| 2. | The swab should be mixed vigerously in the media to release any
Chlamydia organisms collected on it. |
| 3. | 1/10 to 1/2 the liquid media should be placed in a standard 0.5 ml PCR
tube and centrifuged in a microfuge at max RPM to pellet the Chlamydia
organisms. |
| 4. | As much of the supernatant as is possible should be removed. |
| 5. | The pellet from 4 above should then be treated using the standard or
microwave protocols for using GeneReleaser®. |
| 6. | If there is significant volume of liquid remaining on the pellet (10µl or
more) then the liquid volume should be estimated and deducted from
volume of GR used to treat the specimen; however, no less than 5µl of GR
should be used on a given specimen. |
